human adenovirus type 5  (ATCC)

Journal: GMS Hygiene and Infection Control

Article Title: Virucidal efficacy of a UV-C disinfection system for semi-critical medical devices according to EN 14476

doi: 10.3205/dgkh000622

Figure Lengend Snippet: lg TCID 50 /mL titers of Adenovirus Type 5 on glass test surface at initial inoculation, post-drying, and after UV-C treatment

Article Snippet: The following indicator viruses were tested in accordance with EN 14476 requirements: Adenovirus Type 5 (ATCC VR-5), Poliovirus Type 1 LSc-2ab (RVB-1260), Murine Norovirus strain S99 (RVB-651), and Simian Virus 777 (SV40).

Techniques:

Journal: GMS Hygiene and Infection Control

Article Title: Virucidal efficacy of a UV-C disinfection system for semi-critical medical devices according to EN 14476

doi: 10.3205/dgkh000622

Figure Lengend Snippet: lg TCID 50 /mL titers of Adenovirus Type 5 on TPE-test surface at initial inoculation, post-drying, and after UV-C treatment

Article Snippet: The following indicator viruses were tested in accordance with EN 14476 requirements: Adenovirus Type 5 (ATCC VR-5), Poliovirus Type 1 LSc-2ab (RVB-1260), Murine Norovirus strain S99 (RVB-651), and Simian Virus 777 (SV40).

Techniques:

Journal: Materials Today Bio

Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy

doi: 10.1016/j.mtbio.2025.102583

Figure Lengend Snippet: Construction and characterization of CaCO 3 @CM-OA. (A) Particle size analysis of OA, CM-OA, and CaCO 3 @CM-OA. (B) Zeta potential measurements of OA, CM-OA, and CaCO 3 @CM-OA. (C) TEM image of OA and CM-OA, together with SEM image of CaCO 3 @CM-OA. (D) TEM image and corresponding elemental mapping of CaCO 3 @CM-OA. (E, F) Size and PDI of OA, CM-OA, and CaCO3@CM-OA under different pH conditions and time points. (G) Silver-stained SDS-PAGE gel comparing cell membrane, CM-OA, and CaCO 3 @CM-OA. (H) Dot blot assay detecting adenovirus type 5 hexon in OA, CM-OA, and CaCO 3 @CM-OA. (I) Blood and intestinal tissues were collected from mice after oral administration. (J) Relative viral levels in blood at various time points and (K) relative fluorescence intensity in intestinal tissues were quantified. Data are presented as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), p62 (HuaBio, HA721171), Beclin1 (CST, 3495S), LC3A/B (CST, 12741T), p-MLKL (MCE, HY-P81878), MLKL (HuaBio, ET1601-25), N-cadherin (Affinity, AF5239), E-cadherin (Affinity, AF0131), α-SMA (HuaBio, ET1607-53), Vimentin (Affinity, AF7013), Collegen-I (Proteintech, 66761-1-Ig), Collegen-III (Abcam, ab7778).

Techniques: Particle Size Analysis, Zeta Potential Analyzer, Staining, SDS Page, Membrane, Dot Blot, Fluorescence